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NanoMaterials Facility
 Synergy H1m Plate Reader
CNS ID: BIO-13
MakeBioTek
ModelSynergy H1m Monochromator-Based Multi-Mode Microplate Reader
CategoryBiological Sample Prep./Cell Culturing Facility
NameSynergy H1m Plate Reader
LocationLISE - G05
 
 
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More Info
This system supports top and bottom fluorescence intensity, UV-visible absorbance and high performance luminescence detection in a variety of applications, such as: cell viability, growth, toxicity, ELISA, caspase and DNA assays. To create the ideal physiological environment for live-cell assays, this plate reader has a user-adjustable orbital shaking and advanced 4-ZoneTM temperature control (from ambient to 45°C +0.5°C). Optional add-ons:

1. a new Gas Controller for Synergy H1m allows control and monitoring of CO2 and O2 levels within the Synergy H1

2. a dual reagent injection system is available to automate inject/read assays such as ion channels assays or flash luminescence assays (e.g. luciferase or ATP assays).
Comments
Detection mode: Fluorescence, Luminescence, UV-Visible

Absorbance, TRF (secondary mode) Absorbance -microplate types: 1- to 384-well plates Compatible with Take3 plate with 2 µL microspots -wavelength range: 230 - 999 nm, 1 nm increment -dynamic range: 0 - 4.0 OD -resolution: 0.0001 OD

Fluorescence -fluorescence sensitivity: Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) -wavelength selection: Double grating monochromators (Top and Bottom) -wavelength range: 250 - 700 nm (850 nm option) -dynamic range: 5 decades

Luminescence -sensitivity: 20 amol ATP typical (flash) 100 amol ATP (glow) typical -wavelength range 300 - 700 nm -dynamic range >6 decades
 
Contact Info:
Monica Zugravu
LISE G38
11 Oxford Street
Cambridge, MA 02138
617-496-7102
mzugravucns.fas.harvard.edu
 
 

07/23/13
New DRIE on-line and available for Training !!

 

05/02/13
LED Measurement System and Integrating Sphere now available! Contact Jason Tresback for training.

 

03/12/13
Quantox non-contact electrical measurement tool commissioned. See Philippe for training.

 

02/20/13
Olympus Total Internal Reflection Fluorescence Microscopy Hands-On Demo

 

DRIE system on site - available soon.

 

01/18/13
i-line stepper coming to CNS this spring!

 

08/14/12
We bought the k-alpha XPS - it will be staying!

 

05/14/12
We bought the Heidelberg uPG501 direct write system - it will be staying!

 

10/04/12
XeF2 etch system now available!

 

02/21/12
Time Resolved PL Spectroscopy now available