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More Info:
The CNS TIRF Laser Cell Microscope is a Total Internal Reflection Fluorescence Microscope based on Olympus IX70. The 60x TIRF objective provides 90X magnification via the 1.5X tube lens. It is equipped with three lasers: blue DPSS @ 488nm, green DPSS @ 532nm and red HeNe @ 633nm, which are coupled to the microscope. Imaging modalities include bright field epifluorescence, TIRF, Phase Contrast and DIC. Image recording is done with a cooled high-sensitivity EMCCD camera from Roper Scientific with low light level imaging capability.
Click here to watch a video of a TIRF image of 0.3 micron yellow/green spheres excited by a 488nm laser line. Spheres attached to the coverslip are constantly visible whereas other spheres are seen drifting in and out of the field of view via Brownian motion. This demonstrates the superb ~100nm axial resolution available with TIRF microscopy.
Comments:
Total Internal Reflection Fluorescence Microscope
Contact Info:
Arthur McClelland
LISE G40
11 Oxford Street
Cambridge, MA 02138
617-496-8250
amcclellandcns.fas.harvard.edu
Fettah Kosar
LISE G48
11 Oxford St.
Cambridge, MA 02138
617-495-1738
fkosarcns.fas.harvard.edu
cns.fas.harvard.edu
